XF Real-Time ATP Rate Assay well plate set up

Cellular metabolic regulation allows cells to adjust for changes in ATP demand with subsequent changes in ATP production to maintain total intracellular ATP levels. By using the Agilent Seahorse XF Real-Time ATP Rate Assay we can measure total ATP production rates in living cells and to distinguish between the fractions of ATP that are produced from mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, the two main metabolic pathways responsible for ATP production in mammalian cells. 

The XF Real-Time ATP Rate Assay can also be used to calculate the ideal cell seeding density. By creating four different seeding density sources ie 0.5X, 1.0X, 2.0X and 4.0X we can determine which level for our particular cells is best. Optimal cell seeding numbers vary widely, though are typically between 5 × 10³ – 4 × 10⁴ cells per well and must be determined empirically. (For example, for 1 x 10⁴ cells per well, resuspend cells 1 x 10⁴ per 80 μL = 1.25 x 10⁵ cells per mL)

To simultaneously test the ideal seeding level for both Parkinson's and healthy control cells the following well plate design is proposed.

PhD Confirmation ....

Presented my Confirmation paper and presentation on Tuesday 8th September, and it was well received with unanimous agreement to confer me into the PhD program.